Article
Expression stability of six housekeeping genes: A proposal for resistance gene quantification studies of Pseudomonas aeruginosa by real-time quantitative RT-PCR.
Journal of medical microbiology - 1 May 2003
Savli Hakan, Karadenizli Aynur, Kolayli Fetiye, Gundes Sibel, Ozbek Ugur, Vahaboglu Haluk
Abstract excerpt
Constantly expressed genes are used as internal controls in relative quantification studies. Suitable internal controls for such studies have not yet been defined for Pseudomonas aeruginosa. In this study, the genes ampC, fabD, proC, pbp-2, rpoD and rpoS of P. aeruginosa were compared in terms of expression stability by real-time quantitative RT-PCR. A total of 23 strains with diverse resistance phenotypes were...
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