Article
Porin OmpP2 of Haemophilus influenzae shows specificity for nicotinamide-derived nucleotide substrates.
The Journal of biological chemistry - 4 Jul 2003
Andersen Christian, Maier Elke, Kemmer Gabrielle, Blass Julia, Hilpert Anna-Karina, Benz Roland, Reidl Joachim
Abstract excerpt
Haemophilus influenzae has an absolute requirement for NAD (factor V) because it lacks all biosynthetic enzymes necessary for de novo synthesis of that cofactor. Therefore, growth in vitro requires the presence of NAD itself, NMN, or nicotinamide riboside (NR). To address uptake abilities of these compounds, we investigated outer membrane proteins. By analyzing ompP2 knockout mutants, we found that NAD and NMN...
Topics
- Bacterial Outer Membrane Proteins
- Binding Sites
- Biological Transport
- Haemophilus influenzae
- Mutation
- NAD
- Nicotinamide Mononucleotide
- Protein Binding
- Substrate Specificity