Article
Natural transformation of Pseudomonas stutzeri by single-stranded DNA requires type IV pili, competence state and comA.
FEMS microbiology letters - 22 Jan 2002
Meier Petra, Berndt Cecilia, Weger Nicole, Wackernagel Wilfried
Abstract excerpt
Pseudomonas stutzeri, in addition to being transformed by duplex DNA, is also transformed by the sense or antisense strand of the genetic marker employed (hisX(+)) or by heat-denatured chromosomal DNA. Transformation was absent in non-competent cells and in mutants defective for pilus biogenesis (pilA, pilC) and function (pilT) or DNA translocation into the cytoplasm (comA). Uptake of (3)H-thymidine-labeled...
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