Article
N-Glycoproteomic Characterization of Mannosidase and Xylosyltransferase Mutant Strains of Chlamydomonasreinhardtii.
Plant physiology - 1 Mar 2018
Schulze Stefan, Oltmanns Anne, Machnik Nick, Liu Gai, Xu Nannan, Jarmatz Niklas, Scholz Martin, Sugimoto Kazuhiko, Fufezan Christian, Huang Kaiyao, Hippler Michael
Abstract excerpt
At present, only little is known about the enzymatic machinery required for N-glycosylation in Chlamydomonas reinhardtii, leading to the formation of N-glycans harboring Xyl and methylated Man. This machinery possesses new enzymatic features, as C. reinhardtii N-glycans are independent of β1,2-N-acetylglucosaminyltransferase I. Here we have performed comparative N-glycoproteomic analyses of insertional mutants of...
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