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Cloning of the conserved regulatory operon by its aerial mycelium-inducing activity in an amfR mutant of Streptomyces griseus.

Gene - 13 Mar 2003

Komatsu Mamoru, Kuwahara Yoshitaka, Hiroishi Ayako, Hosono Kuniaki, Beppu Teruhiko, Ueda Kenji

Abstract excerpt

We report cloning and characterization of a 2.8 kb DNA fragment that suppressed the aerial mycelium-deficient phenotype of an amfR mutant of Streptomyces griseus when it was introduced on a high-copy-number plasmid. Nucleotide sequencing revealed that the cloned DNA fragment contained a part of a regulatory operon homologous to one of the conserved operons identified in the genome of Streptomyces coelicolor...

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PMID: 12657469
DOI: 10.1016/s0378-1119(03)00405-0

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