Article
Fast purification of the Apo form and of a non-binding heme mutant of recombinant sperm whale myoglobin.
Protein expression and purification - 1 Mar 2003
Ribeiro Euripedes A, Regis Wiliam C B, Tasic Ljubica, Ramos Carlos H I
Abstract excerpt
As molecular biology has developed it has become possible to abundantly produce heterologous proteins in bacteria and to design serial amino acid substitutions for the generation of modified proteins, an approach also known as protein engineering. Sperm whale myoglobin, a protein of broad interest, has been cloned for several years now and a large collection of mutants has been produced. The presence of heme...
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