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Article

Expression, purification, and crystallization of neuro- and cytoglobin.

Methods in enzymology - 1 Jan 2008

Dewilde Sylvia, Mees Kirsten, Kiger Laurent, Lechauve Christophe, Marden Michael C, Pesce Alessandra, Bolognesi Martino, Moens Luc

Abstract excerpt

Neuroglobin and cytoglobin, members of the globin family, are present in vertebrate cells at very low concentrations. As the function of both proteins is still a matter of debate, it is very important to be able to produce and purify these proteins, and in general all members of the globin family, to homogeneity. For this purpose, this chapter describes the expression of neuro- and cytoglobin by E. coli and its...

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PMID: 18237642
DOI: 10.1016/S0076-6879(08)36019-4

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