Article
Heterologous expression and purification of native and mutated low molecular mass glutenin subunits from durum wheat.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences - 25 Mar 2003
Patacchini C, Masci S, D'Ovidio R, Lafiandra D
Abstract excerpt
Wheat technological properties are correlated with the size of glutenin polymers, consisting of high and low molecular mass glutenin subunits, linked together by disulphide bonds. In order to unravel glutenin polymer structure, we considered three LMW-GS genes, which differ in the number of cysteine residues and in the repetitive domain length. The three LMW-GS genes have been expressed in Escherichia coli, and...
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