Full-length genome of wild-type hepatitis A virus (DL3) isolated in China.

Abstract excerpt

AIM: To characterize the genome of an wild-type HAV isolate (DL3) in China. METHODS: A stool specimen was collected from hepatitis A patient from Dalian, China. HAV (DL3) was isolated and viral RNA was extracted. The genome of DL3 was amplified by reverse transcription and polymerase chain reaction (RT-PCR), followed by cloning into pGEM-T vector. The positive colonies were selected and sequenced. The full-length...

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