Engineering of an active animal fatty acid synthase dimer with only one competent subunit.

doi:10.1016/s1074-5521(03)00023-1

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Article

Engineering of an active animal fatty acid synthase dimer with only one competent subunit.

Chemistry & biology - 1 Feb 2003

Joshi Anil K, Rangan Vangipuram S, Witkowski Andrzej, Smith Stuart

Abstract excerpt

Animal fatty acid synthases are large polypeptides containing seven functional domains that are active only in the dimeric form. Inactivity of the monomeric form has long been attributed to the obligatory participation of domains from both subunits in catalysis of substrate loading and condensation reactions. However, we have engineered a fatty acid synthase containing one wild-type subunit and one subunit...

Topics

Acetone
Animals
Blotting, Western
Catalysis
Cross-Linking Reagents
DNA, Complementary
Electrophoresis, Polyacrylamide Gel
Fatty Acid Synthases
Hot Temperature
Indicators and Reagents
Molecular Conformation
Mutation
Protein Engineering

Source

Open in PubMed

Identifiers

PMID: 12618189
DOI: 10.1016/s1074-5521(03)00023-1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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