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Suppression Subtractive Hybridization Identifies Distinctive Expression Markers for Coronary and Internal Mammary Arteries

1 Mar 2003

Abstract excerpt

OBJECTIVE: We sought to identify differentially expressed genes in the athero-prone coronary artery and athero-resistant internal mammary arteries. METHODS AND RESULTS: Using suppressive subtraction hybridization, we generated reciprocal cDNA collections of representative mRNAs specific to porcine coronary arteries versus porcine mammary arteries. We screened 1000 suppressive subtraction hybridization cDNA clones...

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PMID: 12615697
DOI: 10.1161/01.atv.0000059303.94760.5c

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