Insertion mutagenesis of Escherichiacoli GroEL.

Abstract excerpt

To gain insights into the in vivo folding and assembly of bacterial chaperonins, groEL was subjected to insertion mutagenesis using transposon ISlacZ/in. Four GroEL-LacZ fusions and the corresponding insertion mutants were obtained after residues 34, 90, 291, and 367. Apical domain insertion mutants GroEL291 and GroEL367 were degraded into monomeric 30- and 40-kDa fragments, respectively. Only the latter was...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Chaperonin 60
• Share to DNA Transposable Elements
• Share to Escherichia coli