Article
Insertion mutagenesis of Escherichiacoli GroEL.
Biochemical and biophysical research communications - 7 Mar 2003
Amatore Danielle, Baneyx François
Abstract excerpt
To gain insights into the in vivo folding and assembly of bacterial chaperonins, groEL was subjected to insertion mutagenesis using transposon ISlacZ/in. Four GroEL-LacZ fusions and the corresponding insertion mutants were obtained after residues 34, 90, 291, and 367. Apical domain insertion muta...
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