Article
Engineering of bacteriophage T4 tail sheath protein.
Biochemistry. Biokhimiia - 1 Dec 2002
Efimov A V, Kurochkina L P, Mesyanzhinov V V
Abstract excerpt
Gene product 18 (gp18, 659 amino acids) forms bacteriophage T4 contractile tail sheath. Recombinant protein assembles into different length polysheaths during expression in the cell, which complicates the preparation of protein crystals for its spatial structure determination. To design soluble monomeric gp18 mutants unable to form polysheaths and useful for crystallization, we have used Bal31 nuclease for...
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