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Fluorescence lifetime‐based discrimination and quantification of cellular DNA and RNA with phase‐sensitive flow cytometry

20 Feb 2003

Abstract excerpt

BACKGROUND: Simultaneous measurement of cellular DNA and RNA content provides information for determination of the functional status of cells and, clinically, for the diagnosis and grading assessment of various tumors. Most current flow cytometric methods are based on resolving the fluorescence emission spectra of dyes that bind preferentially to either type of nucleic acid. However, several monochromatic nucleic...

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PMID: 12596251
DOI: 10.1002/cyto.a.10022

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