Article
A simple method of DNA extraction and STR typing from urine samples using a commercially available DNA/RNA extraction kit.
Journal of forensic sciences - 1 Jan 2003
Yasuda Toshihiro, Iida Reiko, Takeshita Haruo, Ueki Misuzu, Nakajima Tamiko, Kaneko Yasushi, Mogi Kouichi, Tsukahara Tetsuya, Kishi Koichiro
Abstract excerpt
We devised a simple DNA extraction procedure suitable for STR typing of urine sample. Use of a commercially available DNA/RNA extraction kit equipped with a silica-gel-based membrane made it possible to omit the recovery of urinary nucleated cells by sedimentation before the extraction. Successful genotyping of the TH01, HumTPO and multiplex STRs was achieved using aliquots of urine as small as 100 microL....
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