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A quantitative comparison of urine centrifugation and filtration for the isolation and analysis of urinary nucleic acid biomarkers

13 May 2024

Abstract excerpt

Urine is a rich source of nucleic acid biomarkers including cell-free DNA (cfDNA) and RNA for monitoring the health of kidney allografts. In this study, we aimed to evaluate whether urine filtration can serve as an alternative to the commonly used method of centrifugation to collect urinary fluid and cell pellets for isolating cfDNA and cellular messenger RNA (mRNA). We collected urine specimens from kidney...

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PMID: 38740837
DOI: 10.1038/s41598-024-54239-x

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