Article
Characterization of rpo-B mutant of a strain of Escherichia coli to confirm its suitability for routine work related to recombinant DNA technology.
Roumanian archives of microbiology and immunology - 1 Jan 2000
Nagee A, Mukhopadhyaya P N, Sanyal P K, Kothari I L
Abstract excerpt
Rifampicin resistant spontaneous mutant of a popular laboratory strain of Escherichia coli (DH5 alpha) was isolated and found to resist high level of the drug in growth medium. The growth of the isolate was found to be slower than its wild-type counterpart. Its ability to get transformed into drug-resistant state through transformation by chemical means as tested using plasmid DNA from three different size...
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