Article
[RNA polymerase of a rifampicin-resistant mutant of Escherichia coli has an altered selectivity to phage T7 DNA promoters].
Molekuliarnaia biologiia - 1 Jan 2000
Ozolin' O N, Uteshev T A, Kamzolova S G
Abstract excerpt
The formation of complexes of RNA polymerases from E. coli W12 and its rpoB409 rifampicin resistant mutant with A1 and D promoters of T7 delta D111 DNA was studied by an abortive RNA synthesis technique. The mutation was shown to affect RNA synthesis initiation at these two promotors differentially so that the efficiency of D promotor utilization is enhanced but the use of A1 promotor is unchanged. The mutation...
Topics
- DNA, Viral
- DNA-Directed RNA Polymerases
- Drug Resistance, Microbial
- Escherichia coli
- Kinetics
- Mutation
- Promoter Regions, Genetic
- Rifampin
- T-Phages