Article
[Effects of sigma38 subunit deletion of RNA polymerase on antibiotics biosynthesis in pseudomonas].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Dec 2006
Ge Yi-he, Pei Dong-li, Zhang Xue-hong, Xu Yu-quan
Abstract excerpt
With the designed primers, PCR was carried out using the genomic DNA of Pseudomonas sp. M18 as a template and a 378bp DNA fragment of the rpoS gene was amplified. Then, a 3. 1kb EcoR I -Xho I fragment containing the rpoS gene and its flanking sequence was obtained by screening the genomic DNA library of Pseudomonas sp. M18.A sigma38-subunit-deficient mutant M18S was constructed with insertional gentamycin gene...
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