Article
Chromophore environment provides clue to "kindling fluorescent protein" riddle.
The Journal of biological chemistry - 28 Feb 2003
Chudakov Dmitriy M, Feofanov Alexei V, Mudrik Nikolay N, Lukyanov Sergey, Lukyanov Konstantin A
Abstract excerpt
asCP, the unique green fluorescent protein-like nonfluorescent chromoprotein from the sea anemone Anemonia sulcata, becomes fluorescent ("kindles") upon green light irradiation, with maximum emission at 595 nm. The kindled protein then relaxes to a nonfluorescent state or can be "quenched" instantly by blue light irradiation. In this work, we used asCP mutants to investigate the mechanism underlying kindling....
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