Article
Effects of anti-HPV16E6-ribozyme on phenotype and gene expression of a cervical cancer cell line.
Chinese medical journal - 1 Oct 2002
Zheng Yanfang, Zhang Jiren, Qu Lianghu
Abstract excerpt
OBJECTIVE: To investigate the effects of anti-HPV16E6-ribozyme (HRz) on phenotype and gene expression of a cervical cancer cell line. METHODS: HRz was designed by computer programs. HRz's activity was identified by cleavage experiments in vitro. HRz and empty eukaryotic plasmids were transfected into CaSKi cells with lipofectin, then renamed CaSKi-R and CaSKi-P, respectively. The expression of ribozyme in...
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