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Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set

1 Jan 2002

Abstract excerpt

The design and evaluation of a set of universal primers and probe for the amplification of 16S rDNA from the Domain Bacteria to estimate total bacterial load by real-time PCR is reported. Broad specificity of the universal detection system was confirmed by testing DNA isolated from 34 bacterial species encompassing most of the groups of bacteria outlined in Bergey's Manual of Determinative Bacteriology. However,...

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PMID: 11782518
DOI: 10.1099/00221287-148-1-257

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