Simple and rapid detection of <i>Streptococcus mutans</i> and <i>Streptococcus sobrinus</i> in human saliva by polymerase chain reaction

Abstract excerpt

Streptococcus mutans and Streptococcus sobrinus are major pathogens causing dental caries in humans. A simple and rapid method to detect these species in human saliva simultaneously was developed using the polymerase chain reaction (PCR). Chromosomal DNA was extracted by boiling bacterial cells in lysis solution containing 1% Triton X-100. Oligonucleotide primers specific for portions of the glucosyltransferase...

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