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Article

Quantitative Real-Time PCR for Porphyromonas gingivalis and Total Bacteria

1 Jun 2000

Abstract excerpt

Accurate quantitation of the number of cells of individual bacterial species in dental plaque samples is needed for understanding the bacterial etiology of periodontitis. Real-time PCR offers a sensitive, efficient, and reliable approach to quantitation. Using the TaqMan system we were able to determine both the amount of Porphyromonas gingivalis and the total number of bacterial cells present in plaque samples....

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PMID: 10835003
DOI: 10.1128/jcm.38.6.2362-2365.2000

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