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Article

Development of a novel quantitative real-time assay using duplex mutation primers for rapid detection of Candida species.

Molecular medicine reports - 1 Jan 2012

Xia Qian-Feng, Liu Jin-Bo, Liu Ping, Qin Xi, Qian Shi-Yun, Tu Zhi-Guang

Abstract excerpt

We developed a novel quantitative real-time PCR for quantitating Candida DNA based on the duplex mutation primer principle, in which a signal is generated by melting a duplex mutation primer during renaturation. The duplex mutation primers are much more specific than double-stranded DNA dyes such as SYBR-Green I and, unlike other probes, do not require the double-labeled synthesis of fluorophore and a quencher on...

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PMID: 21964617
DOI: 10.3892/mmr.2011.603

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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