Article
Decrease in cell viability in an RMF, sigma(38), and OmpC triple mutant of Escherichia coli.
Biochemical and biophysical research communications - 29 Nov 2002
Samuel Raj V, Füll Christine, Yoshida Madoka, Sakata Kaori, Kashiwagi Keiko, Ishihama Akira, Igarashi Kazuei
Abstract excerpt
In a speG-disrupted Escherichia coli mutant, which cannot metabolize spermidine to acetylspermidine, addition of spermidine to the medium caused a decrease in cell viability at the late stationary phase of growth. There were parallel decreases in the levels of ribosome modulation factor (RMF), the sigma(38) subunit of RNA polymerase, and the outer membrane protein C (OmpC). To clarify that these three proteins...
Topics
- Bacterial Proteins
- Cell Division
- Centrifugation, Density Gradient
- Dimerization
- Escherichia coli
- Escherichia coli Proteins
- Kinetics
- Mutation
- Porins
- Ribosomal Proteins
- Ribosomes
- Sigma Factor
- Spermidine
