Article
The disulphide mapping, folding and characterisation of recombinant Ber e 1, an allergenic protein, and SFA8, two sulphur-rich 2S plant albumins.
Journal of molecular biology - 15 Nov 2002
Alcocer Marcos J C, Murtagh Gareth J, Bailey Kevin, Dumoulin Mireille, Meseguer Amparo Sarabia, Parker Martin J, Archer David B
Abstract excerpt
We have cloned and expressed genes encoding the allergenic brazil nut 2S albumin (Ber e 1) and the sunflower albumin 8 (SFA8) in the methylotrophic yeast Pichia pastoris. We show that both proteins were secreted at high levels and that the purified proteins were properly folded. We also showed that Ber e 1 is glycosylated during secretion and that the glycan does not interfere with the folding or...
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