Orphan G Protein-Coupled Receptors and Beyond

Abstract excerpt

Since the first discovery of mammalian receptors for adrenaline (beta(2)) and acetylcholine (M(1)) in 1986, many G protein-coupled receptors for known ligands have been cloned by protein purification, PCR (polymerase chain reaction) and low stringency hybridization, and they have been identified by expression cloning techniques. Now we are almost out of the known ligands pool. However, through the achievement of...