Article
Genetic variation in the flanking regions of Shiga toxin 2 gene in Shiga toxin-producing Escherichia coli O157:H7 isolated in Japan.
FEMS microbiology letters - 8 Oct 2002
Hamabata Takashi, Tanaka Tetsuya, Ozawa Ayako, Shima Tatsuichiro, Sato Toshio, Takeda Yoshifumi
Abstract excerpt
We found frequent IS1 integration nearby the stx(2) gene during in vitro mutagenesis of an stx(2) variant, stx(2vhd). To examine the possibility that such insertions have been contributing to generate new stx(2) variants, we screened 86 strains of Escherichia coli O157:H7 isolated in Japan for variations in the ca. 4-kb region flanking the stx(2) locus using PCR methods. Two major classes were identified based on...
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