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Article

Development of a rapid PCR method using the insertion sequence IS1203 for genotyping Shiga toxin-producing Escherichia coli O157.

Journal of clinical microbiology - 1 Dec 2004

Suzuki Masahiro, Matsumoto Masakado, Hata Mami, Takahashi Masao, Sakae Kenji

Abstract excerpt

We developed a rapid PCR method utilizing the diversity of the insertion site IS1203 for genotyping Shiga toxin-producing Escherichia coli (STEC) O157 (IS1203 PCR typing). DNA fragments digested by PvuII, which cut IS1203 at one site, were ligated with themselves and detected by PCR with outward-facing primer pairs for IS1203. To minimize nonspecific bands, nested PCR was also performed. Two fingerprinting...

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Identifiers

PMID: 15583266
DOI: 10.1128/JCM.42.12.5462-5466.2004
PMCID: PMC535262

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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