Analysis of binding sites for the nitrogen-control transcription factor NtcA in the promoters of Synechococcus nitrogen-regulated genes.

Abstract excerpt

Dissociation constant (K(d)) was determined for the binding of the transcriptional regulator NtcA to a number of NtcA-activated promoters of the cyanobacterium Synechococcus sp. PCC 7942, as well as to mutant versions of those promoters altered in their NtcA-binding sites. K(d) values obtained ranged from 27 nM for the NtcA-binding site in the glnA promoter to ca. 1.4 microM for the site in the glnB promoter....

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