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Article

Combination of Dithiothreitol and Detergent Treatment of Spermatozoa Causes Paternal Chromosomal Damage1

1 Nov 2002

Abstract excerpt

Treatment of spermatozoa with either the nonionic detergent Triton X-100 (TX) or dithiothreitol (DTT) has been suggested to confer enhanced success on intracytoplasmic sperm injection (ICSI) in mice and humans. Here, we attempted to use both reagents together, to our knowledge for the first time, and found that this caused severe chromosomal breaks in paternal pronuclei. We documented this effect further by...

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PMID: 12390885
DOI: 10.1095/biolreprod.101.002667

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