Sperm and Oocyte Treatments to Improve the Formation of Male and Female Pronuclei and Subsequent Development Following Intracytoplasmic Sperm Injection into Bovine Oocytes1

Abstract excerpt

This study assessed pronuclear formation, the chromosomal constitution, and the developmental capacity of bovine zygotes formed by intracytoplasmic injection of oocytes with sperm, treated or not with dithiothreitol (DTT). Oocytes were matured in vitro for 22-24 h and then centrifuged so that sperm, prepared by swim-up in the presence or absence of 5 mM DTT, could be injected into the cleared area of the ooplasm....

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