Article
Functional analysis of plastid DNA replication origins in tobacco by targeted inactivation.
The Plant journal : for cell and molecular biology - 1 Oct 2002
Mühlbauer Stefan K, Lössl Andreas, Tzekova Lilia, Zou Zhurong, Koop Hans-Ulrich
Abstract excerpt
Sequences described as chloroplast DNA replication origins were analysed in vivo by creating deletion and insertion mutants via plastid transformation in tobacco. Deletion of the described oriA sequence, which is located within the intron of the trnI gene, resulted in heteroplastomic transformants, when the selection marker was inserted within the intron. Removal of the complete intron sequence together with the...
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