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Rescue of Deletion Mutants to Isolate Plastid Transformants in Higher Plants.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018

El Hajj Mohammad, Hamdan Mohd Fahdli Bin, Avila Elena Martin, Day Anil

Abstract excerpt

Plastid transformation is an attractive alternative to nuclear transformation enabling manipulation of native plastid genes and the insertion of foreign genes into plastids for applications in agriculture and industrial biotechnology. Transformation is achieved using dominant positive selection markers that confer resistance to antibiotics. The very high copy number of plastid DNA means that a prolonged selection...

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PMID: 29987732
DOI: 10.1007/978-1-4939-8654-5_22

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