Article
Temperature-pressure stability of green fluorescent protein: a Fourier transform infrared spectroscopy study.
Biopolymers - 15 Nov 2002
Scheyhing Carsten H, Meersman Filip, Ehrmann Matthias A, Heremans Karel, Vogel Rudi F
Abstract excerpt
Green fluorescent protein (GFP) is widely used as a marker in molecular and cell biology. For its use in high-pressure microbiology experiments, its fluorescence under pressure was recently investigated. Changes in fluorescence with pressure were found. To find out whether these are related to structural changes, we investigated the pressure stability of wild-type GFP (wtGFP) and three of its red shift mutants...
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