Article
Quantifying beta-sheet stability by phage display.
Journal of molecular biology - 6 Sept 2002
Distefano Mark D, Zhong Alan, Cochran Andrea G
Abstract excerpt
The small immunoglobulin G (IgG)-binding protein GB1 is a favored model system for the study of individual residue contributions to the stability of beta-sheets. Nevertheless, only a few of the many possible combinations of mutations have been characterized, leaving many questions unanswered. In order to allow the simultaneous evaluation of libraries of mutants, we have adapted a phage-display method, called...
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