Partial reconstitution of human RNase P in HeLa cells between its RNA subunit with an affinity tag and the intact protein components.

Abstract excerpt

An RNA affinity tag was incorporated into the RNA subunit of human nuclear RNase P. The tagged RNA assembled with the protein components of RNase P inside HeLa cells to generate an active enzyme. Because of the specificity of the RNA tag to streptavidin, the reconstituted complex could be separated from the native enzyme and other ribonucleoproteins (particularly RNase MRP) by streptavidin agarose chromatography...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Affinity Labels
• Share to Blotting, Northern
• Share to Endoribonucleases