Article
Evidence for the Role of Glycosylation in Accessibility of the Extracellular Domains of Human MRP1 (ABCC1)
11 Jul 2002
Abstract excerpt
To enable cell surface localization of the human multidrug resistance protein (MRP1, ABCC1) and to assess the role of the extracellular domains of this transporter, the FLAG epitope tag was introduced into different extracellular loops of the three membrane-spanning domains (MSDs) of the transporter. We constructed and expressed various partially and fully glycosylation-deficient, FLAG-tagged MRP1 proteins in a...
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