Article
Mutation of proline residues in the NH(2)-terminal region of the multidrug resistance protein, MRP1 (ABCC1): effects on protein expression, membrane localization, and transport function.
Biochimica et biophysica acta - 2 Sept 2003
Ito Ken-ichi, Weigl Kevin E, Deeley Roger G, Cole Susan P C
Abstract excerpt
The Multidrug Resistance Protein, MRP1 (ABCC1) confers drug resistance and transports organic anions such as leukotriene C(4) (LTC(4)) and 17beta-estradiol 17-(beta-D-glucuronide) (E(2)17betaG). Previous studies showed that portions of the first membrane spanning domain (MSD1) and the cytoplasmic loop (CL3) connecting it to MSD2 are important for MRP1 transport function. We have replaced 12 prolines in MSD1 and...
Topics
- Alanine
- Cell Membrane
- HeLa Cells
- Humans
- Immunoblotting
- Multidrug Resistance-Associated Proteins
- Mutagenesis, Site-Directed
- Mutation
- Proline
- Protein Transport