Article
Multiple RNA splicing and the presence of cryptic RNA splice donor and acceptor sites may contribute to low expression levels and poor immunogenicity of potential DNA vaccines containing the env gene of equine infectious anemia virus (EIAV).
Veterinary microbiology - 25 Aug 2002
Zhou W, Cook R F, Cook S J, Hammond S A, Rushlow K, Ghabrial N N, Berger S L, Montelaro R C, Issel C J
Abstract excerpt
The env gene is an excellent candidate for inclusion in any DNA-based vaccine approach against equine infectious anemia virus (EIAV). Unfortunately, this gene is subjected to mutational pressure in E. coli resulting in the introduction of stop codons at the 5' terminus unless it is molecularly cloned using very-low-copy-number plasmid vectors. To overcome this problem, a mammalian expression vector was...
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