Article
Two mechanisms for oxidation of cytosolic NADPH by Kluyveromyces lactis mitochondria.
Yeast (Chichester, England) - 1 Jul 2002
Overkamp Karin M, Bakker Barbara M, Steensma H Y, van Dijken Johannes P, Pronk Jack T
Abstract excerpt
Null mutations in the structural gene encoding phosphoglucose isomerase completely abolish activity of this glycolytic enzyme in Kluyveromyces lactis and Saccharomyces cerevisiae. In S. cerevisiae, the pgi1 null mutation abolishes growth on glucose, whereas K.lactis rag2 null mutants still grow on glucose. It has been proposed that, in the latter case, growth on glucose is made possible by an ability of K. lactis...
Topics
- Cell Fractionation
- Cytosol
- Glucose
- Glucose-6-Phosphate Isomerase
- Kluyveromyces
- Mitochondria
- Mutation
- NADP
- NADPH Dehydrogenase
- Oxidation-Reduction
- Oxygen Consumption