Article
Homing at an extragenic locus mediated by VDE (PI-SceI) in Saccharomyces cerevisiae.
Yeast (Chichester, England) - 30 Jun 2002
Nogami Satoru, Fukuda Tomoyuki, Nagai Yuri, Yabe Shizu, Sugiura Masako, Mizutani Ryuta, Satow Yoshinori, Anraku Yasuhiro, Ohya Yoshikazu
Abstract excerpt
PI-SceI (VDE), a homing endonuclease with protein splicing activity, is a genomic parasite in the VMA1 gene of Saccharomyces cerevisiae. In a heterozygous diploid of the VDE-less VMA1 allele and a VDE-containing VMA1 allele, VDE specifically cleaves its recognition sequence (VRS) in the VDE-less VMA1 allele at meiosis, followed by 'homing', i.e. a conversion to a VDE-containing allele. We found that upon VDE...
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