Article
Disruption of Golgi morphology and trafficking in cells expressing mutant prenylated rab acceptor-1.
The Journal of biological chemistry - 27 Sept 2002
Gougeon Pierre-Yves, Prosser Derek C, Da-Silva Lance F, Ngsee Johnny K
Abstract excerpt
Prenylated Rab acceptor (PRA1) is a protein that binds Rab GTPases and the v-SNARE VAMP2. The protein is localized to the Golgi complex and post-Golgi vesicles. To determine its functional role, we generated a number of point mutations and divided them into three classes based on cellular localiz...
Topics
- Animals
- Blotting, Western
- CHO Cells
- Carrier Proteins
- Cell Membrane
- Cricetinae
- Dose-Response Relationship, Drug
- GTP-Binding Proteins
- Golgi Apparatus
- Immunohistochemistry
- Membrane Proteins
- Microscopy, Fluorescence
- Mutagenesis, Site-Directed
- Mutation
- Phenotype
- Point Mutation
- Protein Binding
- SNARE Proteins