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Article

Determinants of the RNase H cleavage specificity of human immunodeficiency virus reverse transcriptase.

Nucleic acids research - 11 Sept 1993

DeStefano J J, Mallaber L M, Fay P J, Bambara R A

Abstract excerpt

We examined the ribonuclease H (RNase H) specificity of human immunodeficiency virus reverse transcriptase (HIV-RT) using heteropolymeric RNAs hybridized to complementary DNAs. Experiments were performed in the presence of excess challenger polymer (poly(rA)-oligo(dT)) to reveal cleavages resulting from single enzyme binding events. Previous results suggested that initial RNase H directed cleavages were a fixed...

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Identifiers

PMID: 7692401
DOI: 10.1093/nar/21.18.4330
PMCID: PMC310068

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