Determinants of the RNase H cleavage specificity of human immunodeficiency virus reverse transcriptase.

doi:10.1093/nar/21.18.4330

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Article

Determinants of the RNase H cleavage specificity of human immunodeficiency virus reverse transcriptase.

Nucleic acids research - 11 Sept 1993

DeStefano J J, Mallaber L M, Fay P J, Bambara R A

Abstract excerpt

We examined the ribonuclease H (RNase H) specificity of human immunodeficiency virus reverse transcriptase (HIV-RT) using heteropolymeric RNAs hybridized to complementary DNAs. Experiments were performed in the presence of excess challenger polymer (poly(rA)-oligo(dT)) to reveal cleavages resulti...

Topics

Base Sequence
DNA, Viral
HIV
Molecular Sequence Data
Mutation
RNA, Viral
RNA-Directed DNA Polymerase
Ribonuclease H
Substrate Specificity

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