Article
Typing of enteroviruses by use of microwell oligonucleotide arrays.
Journal of clinical microbiology - 1 Jun 2009
Susi P, Hattara L, Waris M, Luoma-Aho T, Siitari H, Hyypiä T, Saviranta P
Abstract excerpt
We have developed a straightforward assay for the rapid typing of enteroviruses using oligonucleotide arrays in microtiter wells. The viral nucleic acids are concomitantly amplified and labeled during reverse transcription-PCR, and unpurified PCR products are used for hybridization. DNA strands are separated by alkaline denaturation, and hybridization is started by neutralization. The microarray hybridization...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
