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Article

Characterization of Cu, Zn-superoxide dismutase-deficient mutant of fission yeast Schizosaccharomyces pombe.

Current genetics - 1 May 2002

Mutoh Norihiro, Nakagawa Chiaki W, Yamada Kenichiro

Abstract excerpt

A Cu, Zn-superoxide dismutase gene ( sod1+) deletion mutant of fission yeast Schizosaccharomyces pombe was constructed and its properties were investigated. Superoxide dismutase activity was not detected in the mutant on activity staining of polyacrylamide gels. The mutant showed cysteine or methionine and lysine auxotrophy, slow growth and sensitivity to menadione. While expression of the apt1+ gene, induction...

Topics

Amino Acids
Cell Division
Enzyme Induction
Gene Expression Regulation, Fungal
Hydrogen Peroxide
Mutagenesis
Mutation
Oxidative Stress
Pancreatitis-Associated Proteins
Schizosaccharomyces
Superoxide Dismutase

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PMID: 12073089
DOI: 10.1007/s00294-002-0288-9

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