Article
Differences in rDNA libraries of faecal bacteria derived from 10- and 25-cycle PCRs.
International journal of systematic and evolutionary microbiology - 1 May 2002
Bonnet Régis, Suau Antonia, Doré Joël, Gibson Glenn R, Collins Matthew D
Abstract excerpt
In spite of its shortcoming, analysis of PCR-derived rDNA libraries is being employed increasingly to investigate microbial diversity within many ecosystems. In the present investigation, the effects of the number of PCR cycles (10 vs 25 cycles) on the inferred structure of a 16S rDNA library have been examined. Seventy-five 25-cycle sequences were retrieved and analysed in comparison with 284 10-cycle sequences...
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