Article
Rpf2p, an evolutionarily conserved protein, interacts with ribosomal protein L11 and is essential for the processing of 27 SB Pre-rRNA to 25 S rRNA and the 60 S ribosomal subunit assembly in Saccharomyces cerevisiae.
The Journal of biological chemistry - 9 Aug 2002
Morita Daisuke, Miyoshi Keita, Matsui Yasushi, Toh-E Akio, Shinkawa Hidenori, Miyakawa Tokichi, Mizuta Keiko
Abstract excerpt
Saccharomyces cerevisiae Rrs1p is a nuclear protein that is essential for the maturation of 25 S rRNA and the 60 S ribosomal subunit assembly. In two-hybrid screening, using RRS1 as bait, we have cloned YKR081c/RPF2. Rpf2p is essential for growth and is mainly localized in the nucleolus. The amino acid sequence of Rpf2p is highly conserved in eukaryotes from yeast to human. Similar to Rrs1p, Rpf2p shows physical...
Topics
- Alleles
- Blotting, Northern
- Blotting, Western
- Cell Nucleolus
- Cell Nucleus
- Fluorescent Antibody Technique, Indirect
- Models, Biological
- Plasmids
- Protein Binding
- RNA Precursors
- RNA, Ribosomal
- RNA-Binding Proteins