Neuroblastoma cell-adapted yellow fever 17D virus: characterization of a viral variant associated with persistent infection and decreased virus spread.

Abstract excerpt

Serial passage of yellow fever 17D virus (YF5.2iv, derived from an infectious molecular clone) on mouse neuroblastoma (NB41A3) cells established a persistent noncytopathic infection associated with a variant virus. This virus (NB15a) was dramatically reduced in plaque formation and exhibited impaired replication kinetics on all cell lines examined compared to the parental virus. Nucleotide sequence analysis of...

Topics

• Animals
• Brain
• Chlorocebus aethiops
• Genetic Variation
• Humans
• Mice
• Mutation
• Neuroblastoma
• Tumor Cells, Cultured
• Vero Cells
• Viral Envelope Proteins
• Viral Plaque Assay
• Virulence
• Yellow Fever
• Yellow fever virus